A strain of DHV-N was isolated from liver of duckling, with serum I of duck hepatitis, virus by neutralization test and serum passive immunity protection test, the strain of type I DHV was identified. 使用工型鸭肝炎病毒抗血清,通过中和试验和血清被动免疫保护试验进行鉴定,证明该分离毒株为Ⅰ型DHV。
Then, detected by ELISA in serum specific IgG, intestinal lavage fluid sIgA antibodies, spleen lymphocyte proliferation activity levels and CD4+, CD8+ T lymphocyte typing ratio; and evaluated by animal challenge protection test their immunity. 采用ELISA法分别检测血清中特异性IgG、肠灌洗液中sIgA抗体、脾脏淋巴细胞增殖活性水平及CD4+、CD8+T淋巴细胞分型比值;并通过动物攻毒保护试验对其免疫效果进行评价。